COMET Assay Tank
Full Screen Shot of a Comet Profile within the Comet Assay IV Software
CSL-COM40
COMET10
COMET Assay TankFull Screen Shot of a Comet Profile within the Comet Assay IV SoftwareCSL-COM40COMET10

COMET Assay Tank

Key Features

  • For Single Cell Gel Electrophoresis
  • Minimise exposure to light and reduce background DNA damage
  • High efficiency cooling for enhanced resolution
  • Electrical Safety – lid can be located in one way only. On removal, power is disconnected from buffer chamber
  • View product – in online catalogue
Category: .

Product Description

COMET assay tanks are available in three slide formats to study single cell gel electrophoresis (SCGE), a technique made popular by drug toxicology and carcinogenesis studies for the detection and quantitation of DNA damage in cells. Each tank’s robust construction from ebony acrylic ensures that cells remain free of exposure to background light and DNA damage during electrophoresis, while a cooled central platform provides a convenient surface for slide preparation and control of slide temperature during the assay. Following electrophoresis, DNA damage may be measured using Comet Assay scoring software.

Background: First introduced in 1981 to quantify double-stranded DNA breakages in single cells exposed to g-irradiation, the Comet Assay (or SCGE) has since been adapted to analyse specific DNA lesions and repair processes.
Overview: Following genotoxic insult, such as ionizing radiation, the resultant strand breakage of supercoiled duplex DNA reduces the size of the large genomic DNA from which these strands are separated or drawn out by electrophoresis. The genomic DNA then takes on the appearance of a ‘comet’ as its negatively charged broken ends and fragments migrate towards the anode during electrophoresis.
Method: After exposure to a genotoxic insult cells are suspended within low melting point agarose and embedded within a thin layer of agarose on a microscope slide. Cellular protein is then removed by lysis in detergent, when DNA is allowed to unwind in alkaline conditions before electrophoresis. The DNA is electrophoresed, stained and then analysed using software.
Results: Microscopy imaging is used to measure DNA fluorescence upon staining. In DNA damaged cells the resultant image resembles a ‘comet’ with the cellular DNA separated into a head and tail. The head is mainly composed of intact genomic DNA, whereas any fragmented or damaged DNA is concentrated within and towards the tail.

Ordering Information

BCOM10 Comet Assay Tank for 10 Slides BCOM20 Comet Assay Tank for 20 Slides BCOM40 Comet Assay Tank for 40 Slides

Technical Specification

BCOM10 Comet Assay Tank for 10 Slides
Slide Capacity 10
Unit Dimensions (w x l x h) 17x34x9cm
Volume 550ml
Recommended Running Conditions 25V/300mA for 1h

BCOM20 Comet Assay Tank for 20 Slides
Slide Capacity 20
Unit Dimensions (w x l x h) 31x34x9cm
Volume 1000ml
Recommended Running Conditions 25V/300mA for 1h

BCOM40 Comet Assay Tank for 40 Slides
Slide Capacity 40
Unit Dimensions (w x l x h) 33x59x9cm
Volume 2100ml
Recommended Running Conditions 25V/300mA for 1h

Accessories

CHILLER Chiller unit for active slide temperature control

Downloads

Comet Assay User Manual.pdf – CLICK TO VIEW/DOWNLOAD

Comet Assay catalogue page – CLICK TO VIEW/DOWNLOAD