omniPAGE WAVE Maxi DGGE is a complete system for DNA mutation analysis. Using the innovative vertical screw-clamp technology of the new WAVE Maxi system, the WAVE-DGGE is fully equipped – with temperature control unit, stirrer, gradient mixer and casting accessories – to perform specific mutation analysis applications. The powerful microprocessor-controlled PID temperature control unit enables various mutation detection techniques to be undertaken between ambient temperature and 70°C, while the simple four-screw design of the WAVE insert accelerates set up of denaturing PAGE gels. Accordingly, the new WAVE-DGGE can be used to screen single-base pair changes in the following applications:
• Heteroduplex analysis (HA)
• Parallel Denaturing Gradient Gel Electrophoresis (DGGE)
• Constant Denaturing Gradient Gel Electrophoresis (CDGE)
The flexibility provided by the modular design of the WAVE-DGGE and its wide range of accessories enables laboratories to switch quickly and easily between different mutation detection techniques, thereby maximising throughput and screening efficiency. A maximum 96-sample throughput allows detection of as many mutations within a couple hours, alleviating many of the bottlenecks associated with screening for DNA mutations.
The GM100 gradient mixer is supplied as standard to ensure efficient gradient formation by mixing and delivering high- and low-density denaturant solutions. A flat-base design and support handle allows the GM100 to be secured to a retort stand, enabling it to be easily mounted on a magnetic stirring plate, while the mixing chamber can accommodate a magnetic stirrer to form a linear gradient. The MU-D01 peristaltic pump is also recommended for delivery of linear and reproducible gradient gels.
Innovative Casting and Set-up Mechanism
The WAVE-DGGE utilises novel vertical screw clamp technology to assemble two vertical gels. This reduces the number of screws required for set up making casting assembly faster, while a built-in inner buffer chamber within the PAGE insert allows set-up to be completed without the inclusion of heavy top tanks or buffer chambers. A dual purpose PAGE insert eliminates the need for plate transfer, and is used with a cam casting base to guarantee efficient leak free casting.
Precise thermal control
The redesigned WAVEDGGE-TC temperature control unit combines buffer recirculation with a heat sensor and 1.4kW heating element to facilitate precise temperature control to within ±0.02°C, allowing the gel temperature to be set to the melting temperature (Tm) of the amplified DNA polymorphism or mutation of interest. Other benefits include: a conspicuous 4-digit 16mm LED panel to aid set-up; precise tuning to within 0.1°C resolution; an operating set point, plus three adjustable pre-set temperature values; and stirred buffer circulation for temperature stability and uniformity.
Programmable power supply option
At 500V, 800mA and 300W outputs, the optional MP-500V power supply provides full flexibility for different mutation detection techniques.
Software options: 3 packages
Pre-electrophoresis: mutations are best detected when the single-base pair mutation itself is located within lowest melting domain of the DNA of interest, and the molecule does not denature entirely. GC-clamping at one end of the DNA molecule ensures that the region to be screened is in the lower melting temperature domain while the DNA molecule remains partly double-stranded. Recommended free POLAND analysis software (G Steger, University of Dusseldorf) predicts the melting behaviour of the DNA fragment of interest and resulting primer placement and GC clamp positioning.
Post electrophoresis: Phoretix 1D is available for band pattern matching following parallel DGGE in a single gel; Phoretix 1D Pro allows comparison between multiple gels and different experiments.