CellasGEL ‘wet’ gels

Using CellasGEL

  1. Equilibrate a CellasGEL for 10 minutes in Electrophoresis buffer using an agitating platform (e.g. 3D Shaker)
  2. Dry surplus buffer from the CellasGEL before securing it to a Bridge located within a pre-prepared Cellas tank
  3. Apply samples to the CellasGEL using the appropriate Applicator, and electrophorese at 200V for 30-9 secs (see Power Supplies)
  4. Remove the CellasGEL from the tank, and use the required Clinical Test Kit for staining and destaining and clearing
  5. Place the CellasGEL on a suitably sized mylar sheet or glass plate and dry in an oven for 10 minutes at 80°C (e.g. NHYBRIDBASIC)
  6. Quantify bands using Scanner and Densitometer Software
Category:

Description

CellasGEL ‘wet’ cellulose acetate gel strips are ready to use and overcome many of limitations of traditional ‘dry’ cellulose acetate membranes. CellasGEL’s advantages over dry cellulose acetate membranes are as follows:
1. Wet state – unlike dry membranes, CellasGEL is a cellulose acetate film produced in a wet form to facilitate buffer adsorption, but without the entrapment of air bubbles that inhibit electrophoresis
2. Greater thickness – CellasGEL’s greater thickness (190- 500μm) compared to dry membranes (160-190μm) allows application of larger sample volumes to enhance detection of poor quality specimens low in protein content
3. High resolution – samples may be applied to CellasGEL as wider but finer bands, without risk of diffusion, to make band quantitation more reproducible; this is further enhanced by extended migration distances (60-70mm) that improve band separation
4. Amphiphilic – CellasGEL’s lipophilic and hydrophilic properties make it the perfect separation medium for many different biological molecules, ranging from lipoproteins to haemoglobins CellasGEL is supplied either as individual packs of 25 or 100 strips or within clinical test kits for the following applications:

  • Immunofixation Electrophoresis (IFE): Monoclonal Gammopathies of Undetermined Significance (MGUS); Multiple Myeloma (MM)
  • Serum Protein Analysis: Dysproteinaemia; Incipient Gammopathies
  • Haemoglobin Analysis: Haemoglobinopathies such as Thalassaemias and Sickle Cell Disorders
  • Lipoprotein Analysis: Hyperlipidaemias; High-density (HDL), Low-density (LDL) and Very-low-density (VLDL) lipoprotein evaluation

Applications

CellasGEL Applications Packages equipped with bridges, applicator and clinical test kit Click to view

Ordering Information

KITCU CellasKIT: 1-patient test for serum and concentrated urine IFE
2x semi-micro tests (1x serum and 1x urine) for 1 patient performed on 6x CellasGEL strips (2.5x14cm; 190μm) on 3x bridges in 1x CELLAS
KITI2432 CellasKIT: 6-patient test for serum IFE
6x semi-micro or 8x micro tests (1/patient) on 6x CellasGEL strips (5.7×14 cm; 190μm), performed on 6x bridges in 2x CELLAS units
KITSP100200 CellasKIT: serum proteins
4x semi-micro or 8x micro tests for each CellasGEL strip (5.7x14cm; 200μm); 3 strips / CELLAS* performed on 3x bridges.
KITSP150200 CellasKIT: serum proteins (high resolution)
6x semi-micro or 8x micro tests for each CellasGEL strip (5.7x14cm; 190μm); 3 strips / CELLAS* performed on 3x bridges.
KITHG100 CellasKIT: haemoglobin
4x semi-micro tests for each CellasGEL strip (5.7x14cm; 250μm); 3 strips / CELLAS* performed on 3x bridges
KITLP100 CellasKIT: lipoproteins
4x semi-micro tests for each CellasGEL strip (5.7x14cm; 250μm); 3 strips / CELLAS* performed on 3x bridges

You may also like…