Description
This complete stand-alone unit BCSB10 for 10x10cm electroblotting formats, comprises omniPAGE tank and lid, electroblotting module, cassettes and fibre pads. The electroblotting module includes four inter-locking cassettes and eight fibre pads. A high intensity current is generated by coiled platinum electrodes. These in conjunction with the advanced cooling features allow rapid electroblotting in as little as one hour. Modules are interchangeable with omniPAGE slab and tube gel modules. Hinged rigid cassettes allow rapid set up and convenience and ensure even contact between the gel and membrane is maintained. Each of these techniques benefits from rapid set up cooling packs which provide enhanced resolution even during high intensity 2-D electrophoresis.
Also available is a Hi-Intensity Blot Module. This comprises Plate electrodes, just 2cm apart, to generate a powerful electric field. This provides transfer capability for up to two 10x10cm gels in as little as one hour.
Background
Blotting, a technique that entails immobilisation of proteins or nucleic acids on a solid membrane support and then detection using a specific antibody or probe of complementary nucleic acid sequence, signi cantly increases the potential for identi cation and characterisation of proteins and nucleic acids. Upon transfer to a membrane support proteins and nucleic acids become far more accessible to detection by antibodies and probes than they would otherwise be within a gel. Size-fractionation by gel electrophoresis followed by blotting is an excellent way to identify speci c molecules within a mixed population of nucleic or protein molecules, and the two techniques are often used in tandem.
