Description
The omniPAGE range of Modular Vertical Gel Systems allow multiple electrophoresis techniques to be performed in the same unit. Using the same main tank and lid, three different inserts are interchangeable for PAGE, tube gel and electroblotting techniques. These systems are available in three size options, MINI 10 x 10cm, MINI Wide 20 x 10cm and MAXI WAVE 20 x 20cm, as individual, combination or complete modular systems. Combination and modular systems offer maximum value and space and have the added benefit of increased consistency which results from using the same universal base and lid.
The omniPAGE WAVE complete option provides a fully integrated solution using one universal gel tank and lid for vertical PAGE, tube gel IEF and electroblotting. This concept of interchangeable inserts allows users to combine vertical PAGE with capillary gel IEF to perform 2-D electrophoresis, followed by western transfer for enhanced sensitivity. The supplied detachable internal Cooling Coil allows connection to either the laboratory water supply or a recirculating chiller, and so makes the systems ideal for:
- temperature-sensitive separations and samples and/or
- application of high voltages to achieve precise, high-resolution focusing for pI determination
Blotting
The Standard Platinum Wire module can accommodate up to four 20x20cm, eight 20x10cm or sixteen 10x10cm gels while the optional High-Intensity Plate module facilitates rapid 2-gel transfers.
2D Capillary Electrophoresis
Focus up to 10 first-dimension IEF tube gels within a single run using the dedicated 2-D insert, or use the included blanking ports to run fewer gels if preferred.
Maxi ‘WAVE’ Concept
The new Maxi ‘WAVE’ System is designed to perform a variety of separations, including first- and second-dimension SDS-PAGE, native, preparative, gradient and high-resolution nucleic acid electrophoresis, plus capillary tube gel IEF and electroblotting, the omniPAGE WAVE is one of the most versatile maxi vertical systems available.
The innovative, vertical screw-clamp system within the PAGE insert requires only four screws to secure up to four 20x20cm gels. This gives the omniPAGE WAVE the selective advantage of a much faster set up time compared to products whose traditional clamping configurations require as many as 24 screws to secure just two glass plates. In addition, the WAVE’s innovative vertical screw-clamp configuration distributes pressure evenly along the height of the gel rather than in the centre to eliminate plate bowing and gel compression. This still maintains a leak-proof seal during casting; while the ergonomic wave-like design of he PAGE insert aids both handling and set up. A detachable inner cooling coil connects to the laboratory water supply or a recirculating chiller to provide uniform, smile-free electrophoresis, while allowing runs to be performed at higher voltage. Whether it be running 2 or 4 gels, electroblotting, and IEF using capillary tube gels or IPG strips, all of these techniques may be performed using the same multi-purpose unit. And of course, this is in addition to retaining the benefits of large format electrophoresis, such as extended separation distances, greater sample throughput and superior resolution.
WAVE Maxi TETRAD
A 4-gel omniPAGE WAVE TETRAD system is created simply by introducing additional plates with spacers and appropriate combs. TETRAD is supplied with a casting base and external casting upstand to allow gels to be prepared in advance, ready for the next run.
Interchangeable modules
omniPAGE Maxi blotting and capillary tube gel inserts may be used with the same tank and lid to adapt an WAVE Maxi Standard or Tetrad system for fast, high-quality electroblotting of maxi gels and for reproducible 2-D electrophoresis, respectively. These units are available as stand-alone options or as part of fully integrated electrophoresis systems.
