WAVE Maxi Modular

Key Features

  • Run up to FOUR gels simultaneously [TETRAD systems]
  • Only four screws required to secure glass plates – reduces set up time significantly
  • Vertical screw-clamps distribute pressure evenly along the height of the gel to prevent plate bowing and gel compression
  • Hinged cassettes, with 4 blot capacity
  • 10 tube capacity
  • Rapid set-up electrofocusing
  • Detachable inner cooling coil facilitates rapid and uniform, smile-free electrophoresis, even at higher voltages
  • Injection moulded construction guarantees long life with reliable and consistent performance
  • View product – in online catalogue

Description

The omniPAGE range of Modular Vertical Gel Systems allow multiple electrophoresis techniques to be performed in the same unit. Using the same main tank and lid, three different inserts are interchangeable for PAGE, tube gel and electroblotting techniques. These systems are available in three size options, MINI 10 x 10cm, MINI Wide 20 x 10cm and MAXI WAVE 20 x 20cm, as individual, combination or complete modular systems. Combination and modular systems offer maximum value and space and have the added benefit of increased consistency which results from using the same universal base and lid.

The omniPAGE WAVE complete option provides a fully integrated solution using one universal gel tank and lid for vertical PAGE, tube gel IEF and electroblotting. This concept of interchangeable inserts allows users to combine vertical PAGE with capillary gel IEF to perform 2-D electrophoresis, followed by western transfer for enhanced sensitivity. The supplied detachable internal Cooling Coil allows connection to either the laboratory water supply or a recirculating chiller, and so makes the systems ideal for:

  • temperature-sensitive separations and samples and/or
  • application of high voltages to achieve precise, high-resolution focusing for pI determination

Blotting
The Standard Platinum Wire module can accommodate up to four 20x20cm, eight 20x10cm or sixteen 10x10cm gels while the optional High-Intensity Plate module facilitates rapid 2-gel transfers.
2D Capillary Electrophoresis
Focus up to 10 first-dimension IEF tube gels within a single run using the dedicated 2-D insert, or use the included blanking ports to run fewer gels if preferred.

Maxi ‘WAVE’ Concept
The new Maxi ‘WAVE’ System is designed to perform a variety of separations, including first- and second-dimension SDS-PAGE, native, preparative, gradient and high-resolution nucleic acid electrophoresis, plus capillary tube gel IEF and electroblotting, the omniPAGE WAVE is one of the most versatile maxi vertical systems available.

The  innovative, vertical screw-clamp system within the PAGE insert requires only four screws to secure up to four 20x20cm gels. This gives the omniPAGE WAVE the selective advantage of a much faster set up time compared to products whose traditional clamping configurations require as many as 24 screws to secure just two glass plates. In addition, the WAVE’s innovative vertical screw-clamp configuration distributes pressure evenly along the height of the gel rather than in the centre to eliminate plate bowing and gel compression. This still maintains a leak-proof seal during casting; while the ergonomic wave-like design of he PAGE insert aids both handling and set up. A detachable inner cooling coil connects to the laboratory water supply or a recirculating chiller to provide uniform, smile-free electrophoresis, while allowing runs to be performed at higher voltage. Whether it be running 2 or 4 gels, electroblotting, and IEF using capillary tube gels or IPG strips, all of these techniques may be performed using the same multi-purpose unit. And of course, this is in addition to retaining the benefits of large format electrophoresis, such as extended separation distances, greater sample throughput and superior resolution.

WAVE Maxi TETRAD
A 4-gel omniPAGE WAVE TETRAD system is created simply by introducing­ additional plates with spacers and appropriate combs. TETRAD is supplied with a casting base and external casting upstand to allow gels to be prepared in advance, ready for the next run.

Interchangeable modules
omniPAGE Maxi blotting and capillary tube gel inserts may be used with the same tank and lid to adapt an WAVE Maxi Standard or Tetrad system for fast, high-quality electroblotting of maxi gels and for reproducible 2-D electrophoresis, respectively. These units are available as stand-alone options or as part of fully integrated electrophoresis systems.

Ordering Information

BCWAVECES Complete WAVE Maxi System for PAGE, 2-D electrophoresis and blotting, comprising: 1x WAVE Maxi Vertical Unit which includes: PAGE insert, 2x4mm thick notched glass plates, 2x4mm thick plain glass plates with 1mm thick bonded spacers, 1x dummy plate, 2x combs (1mm thick 24 samples), 1x casting base, silicone mat, cooling coil;
plus: 1x WAVE Standard Electroblotting Module which includes: WAVE Maxi Platinum Wire Blotting Insert, 4x compression cassettes and 16x fibre pads;
and: 1x WAVEDCI Capillary Electrophoresis Module which includes: WAVE Maxi Tube Gel Insert, 100x capillary tubes,10x blanking ports; and 1x WAVE IEF-KIT: 1 set of plain glass plates with bonded spacers, 0.6x20cm (WxH); and 2x 2-D combs with one 3.5mm marker lane and one 18cm preparatory well.

Technical Specification

Number of gels: 1 – 4
Plate dimensions: 20 x 20 x 0.4cm (W x H x T)
Standard Spacer Dimensions: 2 x 20cm IPG (W x H)
Spacer Dimensions: 0.6 x 20cm(W x H)
Total Volume Inner Buffer Chamber: 640ml
Total buffer volume for 2 gels: 5.3L
Total buffer volume for 4 gels: 4.8L
Standard run time for SDS-PAGE
without cooling: 4 – 5 hours
with cooling: 3 – 4 hours
Max. sample capacity:
PAGE – 192 samples, 48/gel
Blot – 4 blots
2D – 10 tubes
Unit Dimensions: 30 x 18 x 27cm (W x D x H)
Weight: 2.5kg

Accessories

Combs available (click to view):
No. of samples 1, 5, 10, 18MC, 24, 30, 36MC, 48
Thicknesses 0.75, 1, 1.5, 2mm

VS20WAVE-EC VS20 WAVE External Casting Stand – No Casting Base
VS20WAVECAST VS20 WAVE Casting Base
VS20WAVEDIRM VS20 WAVE PAGE insert
VS20WAVE-CC Detachable Cooling Coil
VS20DCAST omniPAGE WAVE Maxi, 20 x 20cm Dual Caster
VS20DCASTM Replacement Rubber mats for 20 x 20cm Caster
VS20DIRM omniPAGE WAVE Maxi – Inner Running Module
VS20ICB WAVE Maxi Cooling Pack
VS20-x -LG Loading guides for omniPAGE WAVE Maxi combs, x = comb well number
VS20NG 20 x 20cm Notched Glass Plates 4mm thick (pk/2)
VS20PG 20 x 20cm Plain Glass Plates 4mm thick (pk/2)
VS20NGS0.75 20 x 20cm Notched Glass Plates with 0.75mm Bonded Spacers (pk/2)
VS20PGS0.75 20 x 20cm Plain Glass Plates with 0.75mm Bonded Spacers (pk/2)
VS20NGS1 20 x 20cm Notched Glass Plates with 1mm Bonded Spacers (pk/2)
VS20PGS1 20 x 20cm Plain Glass Plates with 1mm Bonded Spacers (pk/2)
VS20PGS1.5 20 x 20cm Plain Glass Plates with 1.5mm Bonded Spacers (pk/2)
VS20PGS2 20 x 20cm Plain Glass Plates with 2mm Bonded Spacers (pk/2)
VS20DP Dummy Plate, 20 x 20cm
VS20S0.75 20cm Spacers – 0.75mm (pk/2)
VS20S1 20cm Spacers – 1mm thick (pk/2)
VS20S1.5 20cm Spacers – 1.5mm thick (pk/2)
VS20S2 20cm Spacers – 2mm thick (pk/2)
RPW-0.2100 Replacement Platinum Wire – 0.2mm, 100cm

VS20BI WAVE Maxi Platinum Wire Blot Module – 4 cassettes and 16 fibre pads
SW20BI-HI WAVE Maxi Hi-Intensity Blot Module – 1x cassette and 8 fibre pads
SW20C WAVE Maxi Blot Cassette
SW20F Fibre pads – pk/8

BCVS20DCI WAVE Maxi Tube Gel Module – includes glass tubes and blanking ports
MCT20 Maxi Capillary Tubes, 1.0mm, pk/10
MCT201.5 Maxi Capillary Tubes, 1.5mm, pk/10
MCBP Maxi Capillary Blanking ports

Downloads

omniPAGE WAVE Maxi Systems User Manual – CLICK TO VIEW/DOWNLOAD

omniPAGE WAVE Maxi catalogue page – CLICK TO VIEW/DOWNLOAD

omniPAGE Packages – CLICK TO VIEW/DOWNLOAD

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