Powdered & Liquid Buffers

Key Features

Available in both powder and liquid form
Dry TBE is supplied in packs for extended shelf life
Each sachet will make 1 litre of working solution
Convenience – as ready-made 50x TAE and 10x TBE solutions in 1 and 5 litre volumes
View product – in online catalogue

Description

TBE and TAE are available in both powder and liquid form. Both TBE and TAE are widely used to separate nucleic acids by horizontal gel electrophoresis, and occasionally in vertical polyacrylamide gels. TBE has a higher buffering capacity than TAE, which is used for faster separations of linear double-stranded DNA. Dry TBE is supplied in packs of 10 powder sachets to maintain shelf life. Each buffer sachet may be opened as required and reconstituted in distilled water to make 1 litre of working solution. Buffers are also provided as ready-made 50x TAE and 10x TBE solutions in 1 and 5 litre volumes. These are ideal for laboratories running horizontal nucleic acid gels on a daily basis that require off-the-shelf working stock solutions.

Ordering Information

TBEP Powdered Tris-Borate-EDTA Running Buffer – 10 sachets (1 litre / pack)
TBE10X1L Buffer Tris-Borate-EDTA Running Buffer, 10 x 1L
TBE10X5 Buffer Tris-Borate-EDTA Running Buffer, 10 x 5L
TAE50X1L Buffer Tris-Acetate-EDTA Running Buffer, 50 x 1L
TAE50X5L
Buffer Tris-Acetate-EDTA Running Buffer, 50 x 5L

Technical Specification

TAE Each 50x solution contains: Tris-base (2.0M final stock concentration); glacial acetic acid (1.0M); EDTA, pH 8.0 (0.05M); followed by distilled water to 1L

TBE Each 10x solution / powder contains: Tris-base (0.112M final stock concentration); boric acid (0.112M); EDTA, pH 8.0 (0.02M); followed by distilled water to 1L