Description
TBE and TAE are available in both powder and liquid form. Both TBE and TAE are widely used to separate nucleic acids by horizontal gel electrophoresis, and occasionally in vertical polyacrylamide gels. TBE has a higher buffering capacity than TAE, which is used for faster separations of linear double-stranded DNA. Dry TBE is supplied in packs of 10 powder sachets to maintain shelf life. Each buffer sachet may be opened as required and reconstituted in distilled water to make 1 litre of working solution. Buffers are also provided as ready-made 50x TAE and 10x TBE solutions in 1 and 5 litre volumes. These are ideal for laboratories running horizontal nucleic acid gels on a daily basis that require off-the-shelf working stock solutions.
