runVIEW is an innovative new system which can be used to maximise the efficiency of DNA recovery from runSAFE, EtBr and SYBR stained gels by minimising the number of steps involved in postelectrophoretic purification. runVIEW consists of an multiSUB CHOICE gel chamber with special bluVIEW lid, containing an orange spectral emission filter within its viewing pane, plus a base unit with integrated power supply and blue LED gel illuminator.
Simple DNA recovery
Using runVIEW for gel-free DNA recovery is simple:
1. Cast a gel with two identical rows of wells using one of the matching pairs of 3mm combs supplied. Transfer to the tank, remove the combs, and add buffer just to cover the gel and fill the wells. Load DNA (‘loading tier’).
2. Replace the lid and apply the power. Turn on the blue LED and see the DNA samples migrate in real-time.
3. When the DNA bands of interest enter the second row of wells (‘extraction tier’), stop the power, remove lid and harvest the DNA by pipette.
Once the DNA bands of interest enter the ‘extraction’ tier, simply stop the power supply, remove the lid and harvest the DNA by pipette.
Track DNA without harmful UV
Blue light is safe and has none of the detrimental effects of UV transillumination, including mutagenesis which can compromise cloning efficiency, while runVIEW’s capacity to provide real-time visualisation of electrophoresis enables DNA to be tracked as it migrates through the gel. This allows the user to judge precisely when the band of interest is ready for extraction.
Save on time-consuming gel elution techniques
Running DNA into a well containing buffer effectively purifies the DNA of agarose, eliminating the need for time-consuming gel excision and purification techniques that also result in sample loss. Once harvested, the DNA needs only to be cleaned by a straightforward ethanol precipitation when it is ready for further digestion or ligation.
No expensive commercial gels
runVIEW works with standard EtBr, SYBR Green and SYBR Safe gels cast within the 15×7, 15×10 or 15x15cm CHOICE gel trays, and therefore does not require expensive precast gels and accessories.
A self-contained system
The base unit, which houses the in-built power supply and blue LED gel illuminator, is compact, dual-voltage and portable, and allows electrophoresis, gel visualisation and extraction to be performed at the bench, without the inconvenience of having to transport gels to a darkroom elsewhere within the laboratory.