Biocom Semi Dry Blotters offer rapid transfer times for DNA, RNA and protein blotting – typically 15 to 30 minutes. All units can be used for all types of blotting: western, southern and northern via uncomplicated buffer and set up procedures and are compatible with gel thicknesses from 0.25 up to 10mm without the need for additional equipment. Each unit is compatible with their respective omniPAGE vertical gel system.
Semi Dry Blotting has the added benefit of economic transfers due to very low buffer volumes – typically only a few millilitres of buffer are required per transfer. These Semi-Dry Blotters utilise a screw down lid, which secures the blot sandwich and allows complete control of pressure ensuring even transfer. The electrodes, comprising platinum coated anode and stainless steel cathode, will exhibit practically no corrosion and so provide many years of trouble free use.
Uniform heat dispersion across the blot sandwich ensures stable transfer times and no heat induced sample loss or transfer distortions. Being translucent, it allows viewing of the blot sandwich to ensure correct positioning and transfer is occurring correctly. Electrode plates are fully separated to prevent arching or damage.
Blotting, a technique that entails immobilisation of proteins or nucleic acids on a solid membrane support and then detection using a specific antibody or probe of complementary nucleic acid sequence, signi cantly increases the potential for identi cation and characterisation of proteins and nucleic acids. Upon transfer to a membrane support proteins and nucleic acids become far more accessible to detection by antibodies and probes than they would otherwise be within a gel. Size-fractionation by gel electrophoresis followed by blotting is an excellent way to identify speci c molecules within a mixed population of nucleic or protein molecules, and the two techniques are often used in tandem.