omniPAGE WAVE Blotting Systems include all necessary components for blotting of vertical slab gels. Interchangeable modular inserts combine vertical PAGE with electrotransfer using the same universal tank and lid. The Hi-Intensity electroblotting module with coiled platinum plate electrodes, just 4cm apart are perfect for rapid transfers – 1-2 hours, at 200V. A detachable cooling coil, which connects to the laboratory water supply or a recirculating chiller, prevents buffer depletion and so allows overnight transfers while also maintaining the low temperatures important for protein stability during native transfers and also for fast high-intensity blots.
The standard electroblotting module, available as an option, is ideal for optimum capacity. With platinum wire electrodes 8cm apart, it includes four inter-locking cassettes and eight fibre pads, providing capacity for up to 4 WAVE Maxi gels, 8 omniPAGE Mini Wide or 16 omniPAGE Mini gels – at voltages as low as 35V
Complete stand-alone units for electroblotting, BCSW20-Hi comprise omniPAGE tank and lid, electroblotting module, cassettes and fibre pads. Modules are interchangeable with omniPAGE slab and tube gel modules. Hinged rigid cassettes allow rapid set up and convenience and ensure even contact between the gel and membrane is maintained. An optional High-Intensity Plate module facilitates rapid 2-gel transfers.
Maxi ‘WAVE’ Concept
The new Maxi ‘WAVE’ System is designed to perform a variety of separations, including first- and second-dimension SDS-PAGE, native, preparative, gradient and high-resolution nucleic acid electrophoresis, plus capillary tube gel IEF and electroblotting, the omniPAGE WAVE is one of the most versatile maxi vertical systems available.
The innovative, vertical screw-clamp system within the PAGE insert requires only four screws to secure up to four 20x20cm gels. This gives the omniPAGE WAVE the selective advantage of a much faster set up time compared to products whose traditional clamping configurations require as many as 24 screws to secure just two glass plates. In addition, the WAVE’s innovative vertical screw-clamp configuration distributes pressure evenly along the height of the gel rather than in the centre to eliminate plate bowing and gel compression. This still maintains a leak-proof seal during casting; while the ergonomic wave-like design of he PAGE insert aids both handling and set up. A detachable inner cooling coil connects to the laboratory water supply or a recirculating chiller to provide uniform, smile-free electrophoresis, while allowing runs to be performed at higher voltage. Whether it be running 2 or 4 gels, electroblotting, and IEF using capillary tube gels or IPG strips, all of these techniques may be performed using the same multi-purpose unit. And of course, this is in addition to retaining the benefits of large format electrophoresis, such as extended separation distances, greater sample throughput and superior resolution.
WAVE Maxi TETRAD
A 4-gel omniPAGE WAVE TETRAD system is created simply by introducing additional plates with spacers and appropriate combs. TETRAD is supplied with a casting base and external casting upstand to allow gels to be prepared in advance, ready for the next run.
omniPAGE Maxi blotting and capillary tube gel inserts may be used with the same tank and lid to adapt an WAVE Maxi Standard or Tetrad system for fast, high-quality electroblotting of maxi gels and for reproducible 2-D electrophoresis, respectively. These units are available as stand-alone options or as part of fully integrated electrophoresis systems.