Designed primarily for wet electroblotting of proteins, EBlot Electroblotters offer a combination of increased capacity with economy saving features. Both units, Mini 10 x 10cm and Maxi 20 x 20cm, have increased capacity over standard systems with up to five gel blot cassettes utilised at any one time. This is especially useful in high throughput laboratories.
A uniform electric field is provided by a high intensity coiled electrode and ensures uniform transfer across the blot surface. The cassette’s open architecture ensures the maximum blot area allows direct transfer of current. Its rigid construction ensures contact between the gel and membrane is retained throughout the blot and an even pressure is maintained. These units are compatible with magnetic stirrers to aid heat dispersal and prevent pH drifts in the buffer due to incomplete buffer mixing. Each system includes a cooling pack to further enhance transfer efficiency by removing excess heat. This also saves on buffer for added economy.
Blotting, a technique that entails immobilisation of proteins or nucleic acids on a solid membrane support and then detection using a specific antibody or probe of complementary nucleic acid sequence, signi cantly increases the potential for identi cation and characterisation of proteins and nucleic acids. Upon transfer to a membrane support proteins and nucleic acids become far more accessible to detection by antibodies and probes than they would otherwise be within a gel. Size-fractionation by gel electrophoresis followed by blotting is an excellent way to identify speci c molecules within a mixed population of nucleic or protein molecules, and the two techniques are often used in tandem.